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recombinant human metalloproteinase mmp  (R&D Systems)


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    R&D Systems recombinant human metalloproteinase mmp
    Recombinant Human Metalloproteinase Mmp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mmp%E2%80%9113/Recombinant+Human+MMP-13+Protein%2C+CF/us12589163-1159-1-8
    Average 94 stars, based on 41 article reviews
    recombinant human metalloproteinase mmp - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Shedding blocking agents with increased stability
    Article Snippet: Metalloproteinases—Commercial recombinant human metalloproteinase MMP-2 was used both from Anaspec (Cat. No. AS-72005) or R&D system (Cat. No. 902-MP). .. Commercial recombinant human metalloproteinase MMP-13 was purchased from R&D system (Cat. No. 511-MM). ..

    Article Title: Small shedding blocking agents
    Article Snippet: Metalloproteinases—Commercial recombinant human metalloproteinase MMP-2 was used both from Anaspec (Cat. No. AS-72005) or R&D system (Cat. No. 902-MP). .. Commercial recombinant human metalloproteinase MMP-13 was purchased from R&D system (Cat. No. 511-MM). ..

    Cell Culture:

    Article Title: Dermofunctional Vehicle Downregulates LL-37 and MMPs and Upregulates IGFBP-3.
    Article Snippet: For the RT-qPCR assay, total RNA extraction was followed by cDNA synthesis using the SuperScript® III RT kit (Invitrogen, Carlsbad, CA, USA). .. Cytokine, metalloproteinase, IGFBP-3, and LL-37 measurements in cell culture supernatants were performed using specific ELISA kits for IL-6, TNF-α, TGF-β, IL-10, MMP-1, MMP-3, and MMP-13 (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s instructions. .. Transwell chambers (Corning, Corning, NY, USA) were used for co-cultures, and all other chemicals were of analytical grade supplied by Sigma-Aldrich (St. Louis, MO, USA), unless otherwise indicated.

    Article Title: Dermofunctional Vehicle Downregulates LL-37 and MMPs and Upregulates IGFBP-3
    Article Snippet: For the RT-qPCR assay, total RNA extraction was followed by cDNA synthesis using the SuperScript ® III RT kit (Invitrogen, Carlsbad, CA, USA). .. Cytokine, metalloproteinase, IGFBP-3, and LL-37 measurements in cell culture supernatants were performed using specific ELISA kits for IL-6, TNF-α, TGF-β, IL-10, MMP-1, MMP-3, and MMP-13 (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s instructions. .. Transwell chambers (Corning, Corning, NY, USA) were used for co-cultures, and all other chemicals were of analytical grade supplied by Sigma-Aldrich (St. Louis, MO, USA), unless otherwise indicated.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Dermofunctional Vehicle Downregulates LL-37 and MMPs and Upregulates IGFBP-3.
    Article Snippet: For the RT-qPCR assay, total RNA extraction was followed by cDNA synthesis using the SuperScript® III RT kit (Invitrogen, Carlsbad, CA, USA). .. Cytokine, metalloproteinase, IGFBP-3, and LL-37 measurements in cell culture supernatants were performed using specific ELISA kits for IL-6, TNF-α, TGF-β, IL-10, MMP-1, MMP-3, and MMP-13 (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s instructions. .. Transwell chambers (Corning, Corning, NY, USA) were used for co-cultures, and all other chemicals were of analytical grade supplied by Sigma-Aldrich (St. Louis, MO, USA), unless otherwise indicated.

    Article Title: Effect of type I collagen on TLR-3 induced MMP-13 expression in human periodontal ligament fibroblasts
    Article Snippet: .. Quantification of pro-MMP-13 was conducted using the Human Pro-MMP-13 Quantikine® ELISA Kit (R&D Systems, USA). .. The optical density (OD) of each well was measured at 450 nm using a microplate reader (MultiskanTM FC Microplate Photometer, Thermo Scientific, USA).

    Article Title: Dermofunctional Vehicle Downregulates LL-37 and MMPs and Upregulates IGFBP-3
    Article Snippet: For the RT-qPCR assay, total RNA extraction was followed by cDNA synthesis using the SuperScript ® III RT kit (Invitrogen, Carlsbad, CA, USA). .. Cytokine, metalloproteinase, IGFBP-3, and LL-37 measurements in cell culture supernatants were performed using specific ELISA kits for IL-6, TNF-α, TGF-β, IL-10, MMP-1, MMP-3, and MMP-13 (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s instructions. .. Transwell chambers (Corning, Corning, NY, USA) were used for co-cultures, and all other chemicals were of analytical grade supplied by Sigma-Aldrich (St. Louis, MO, USA), unless otherwise indicated.

    Article Title: Effect of type I collagen on TLR-3 induced MMP-13 expression in human periodontal ligament fibroblasts
    Article Snippet: .. Quantification of pro-MMP-13 was conducted using the Human Pro-MMP-13 Quantikine® ELISA Kit (R&D Systems, USA). .. The optical density (OD) of each well was measured at 450 nm using a microplate reader (MultiskanTM FC Microplate Photometer, Thermo Scientific, USA).

    Binding Assay:

    Article Title: Compound useful for the treatment of degenerative and inflammatory diseases
    Article Snippet: .. For this purpose, 384 well plates (NUNC, 460518, MaxiSorb black) were coated with 35 μL of a 1.5 μg/mL anti-human MMP13 antibody (R&D Systems, MAB511) solution for 24 hrs at 4° C. After washing the wells 2 times with PBS+0.05% Tween, the remaining binding sites were blocked with 100 μL 5% non-fat dry milk (Santa Cruz, sc-2325, Blotto) in PBS for 24 hr at 4° C. Next, the wells were washed twice with PBS+0.05% Tween and 35 μL of 1/10 dilution of culture supernatant containing MMP13 in 100-fold diluted blocking buffer was added and incubated for 4 hr at room temperature. .. Next the wells were washed twice with PBS+0.05% Tween followed by MMP13 activation by addition of 35 μL of a 1.5 mM 4-Aminophenylmercuric acetate (APMA) (Sigma, A9563) solution and incubation at 37° C. for 1 hr.

    Blocking Assay:

    Article Title: Compound useful for the treatment of degenerative and inflammatory diseases
    Article Snippet: .. For this purpose, 384 well plates (NUNC, 460518, MaxiSorb black) were coated with 35 μL of a 1.5 μg/mL anti-human MMP13 antibody (R&D Systems, MAB511) solution for 24 hrs at 4° C. After washing the wells 2 times with PBS+0.05% Tween, the remaining binding sites were blocked with 100 μL 5% non-fat dry milk (Santa Cruz, sc-2325, Blotto) in PBS for 24 hr at 4° C. Next, the wells were washed twice with PBS+0.05% Tween and 35 μL of 1/10 dilution of culture supernatant containing MMP13 in 100-fold diluted blocking buffer was added and incubated for 4 hr at room temperature. .. Next the wells were washed twice with PBS+0.05% Tween followed by MMP13 activation by addition of 35 μL of a 1.5 mM 4-Aminophenylmercuric acetate (APMA) (Sigma, A9563) solution and incubation at 37° C. for 1 hr.

    Incubation:

    Article Title: Compound useful for the treatment of degenerative and inflammatory diseases
    Article Snippet: .. For this purpose, 384 well plates (NUNC, 460518, MaxiSorb black) were coated with 35 μL of a 1.5 μg/mL anti-human MMP13 antibody (R&D Systems, MAB511) solution for 24 hrs at 4° C. After washing the wells 2 times with PBS+0.05% Tween, the remaining binding sites were blocked with 100 μL 5% non-fat dry milk (Santa Cruz, sc-2325, Blotto) in PBS for 24 hr at 4° C. Next, the wells were washed twice with PBS+0.05% Tween and 35 μL of 1/10 dilution of culture supernatant containing MMP13 in 100-fold diluted blocking buffer was added and incubated for 4 hr at room temperature. .. Next the wells were washed twice with PBS+0.05% Tween followed by MMP13 activation by addition of 35 μL of a 1.5 mM 4-Aminophenylmercuric acetate (APMA) (Sigma, A9563) solution and incubation at 37° C. for 1 hr.



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    Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 <t>and</t> <t>MMP-13</t> in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.
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    Image Search Results


    Efficacy of mechanically sensitized OBNC microspheres in the treatment of osteoarthritis. A) Diagram of OBNC microspheres self-adaptive filling of a cartilage injury site to promote cartilage repair and regeneration. B) Representative images of H&E staining. C) OARSI scores of articular cartilage in each group. D) Representative images of safranin O-fast green staining. E) Relative glycosaminoglycan (GAG) content in each group. F) Representative sections showing TUNEL staining for apoptotic cells. G) Quantification of TUNEL-positive cells. H) Representative sections showing Collagen II staining for chondrocytes. I) Relative collagen II expression in each group. J) Representative sections showing MMP-13 staining of chondrocytes. K) Relative MMP-13 expression in each group. (ns: non-significant, ∗ P < 0.05, ∗∗ P < 0.01).

    Journal: Bioactive Materials

    Article Title: Mechanically sensitized hydrogel microspheres trigger membrane receptor switch for cartilage repair

    doi: 10.1016/j.bioactmat.2026.03.017

    Figure Lengend Snippet: Efficacy of mechanically sensitized OBNC microspheres in the treatment of osteoarthritis. A) Diagram of OBNC microspheres self-adaptive filling of a cartilage injury site to promote cartilage repair and regeneration. B) Representative images of H&E staining. C) OARSI scores of articular cartilage in each group. D) Representative images of safranin O-fast green staining. E) Relative glycosaminoglycan (GAG) content in each group. F) Representative sections showing TUNEL staining for apoptotic cells. G) Quantification of TUNEL-positive cells. H) Representative sections showing Collagen II staining for chondrocytes. I) Relative collagen II expression in each group. J) Representative sections showing MMP-13 staining of chondrocytes. K) Relative MMP-13 expression in each group. (ns: non-significant, ∗ P < 0.05, ∗∗ P < 0.01).

    Article Snippet: Antigen retrieval was performed by microwaving in citrate buffer for 10 min. Endogenous peroxidase activity was blocked by incubating the sections with 3% hydrogen peroxide for 10 min, and non-specific binding was blocked with 3% BSA for 1 h. The sections were incubated overnight at 4 °C with primary antibodies to Collagen II and MMP-13 (ServiceBio, China), washed, and incubated for 1 h with HRP-labeled secondary antibodies (ServiceBio, China).

    Techniques: Staining, TUNEL Assay, Expressing

    Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.

    Journal: Non-coding RNA Research

    Article Title: Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression

    doi: 10.1016/j.ncrna.2025.11.007

    Figure Lengend Snippet: Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.

    Article Snippet: Sections were then incubated with primary antibodies against Collagen II (28459-1-AP; 1:200; Proteintech, USA), Aggrecan (13880-1-AP; 1:100; Proteintech), MMP-3 (17873-1-AP; 1:200; Proteintech), and MMP-13 (18165-1-AP; 1:100; Proteintech), followed by the corresponding secondary antibodies.

    Techniques: In Vivo, Staining, TUNEL Assay, Immunohistochemistry, Expressing